Using Next-Generation Sequencing (NGS) to Improve Sexual Assault Investigations Workshop

Thursday November 3rd, 2022 // 1:00 pm - 4:00 pm

Early Registration$200.00
Standard Registration$220.00
Virtual Registration$125.00


Sexual assault kits make up the majority of operational casework for many laboratories in the United States. Next-generation sequencing offers enhanced technical and analytical capabilities that can aid in processing these kits effectively. This workshop explores how NGS can obtain more information, provide more investigative leads and thus reduce the backlog. A panel of experts will discuss (1) the strengths and limitations of CE and NGS, (2) the value of Y-STRs and SNPs for short and long range familial searches, (3) options for STR and SNP mixture deconvolution, and (4) data interpretation and validation studies to enable seamless implement NGS for sexual assault resolution.


Learning Outcomes:

  1. Understand the relative benefits and limitations of CE and NGS for processing sexual assault samples in an operational laboratory setting
  2. Learn how novel markers and techniques can be employed in analysis of sexual assault casework
  3. Discover what mixture deconvolution software options are available for the different markers available with NGS
  4. How other labs have evaluated or validated NGS for their sexual assault casework workflows

Handouts include development validation studies and implementation strategies.


Intended Audience:

Lab directors, Technical Leaders, Forensic scientists, Criminalists


Bruce BudowleChair

Director, Center for Human Identification

Dr. Budowle is the Director of the Center for Human Identification and Professor at the University of North Texas Health Science Center at Fort Worth, Texas. His current efforts focus on the areas of human forensic identification, microbial forensics, and emerging infectious disease with substantial effort in next generation sequencing. He is a Commissioner on the Texas Forensic Science Commission.

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Rachel Oefelein

DNA Labs International (DLI)

Rachel Oefelein is currently the Director of Research and Innovation, Quality Assurance Manager, and a Senior DNA Analyst at DNA Labs International (DLI). Since joining DNA Labs International in 2014, Rachel has testified in eleven Florida counties, five states and U.S. Territories, and five countries as an expert witness for both the prosecution and the defense in misdemeanor and felony trials, as well as Williams, Daubert, and Arthur hearings.

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